Software & Data
Software and data related to our publications can be found at: Github repositories
Microscopy
We have the following setups:
Abberior STED (Confocal Super-resolution)
Olympus IX83 inverted stand with perfect focus. 4 excitation lasers + 775 nm depletion laser compatible for 2-color STED. Includes a PicoQuant module offering FCS, FLIM, and homo-FRET capabilities.
ASI SCAPE (3D Tissue Dynamics)
Applied Scientific Instrument (ASI) design. 3 excitation lasers with dual-camera Kinetix22 for wide FOV and ultra-fast gentle volumetric imaging. Includes temperature and CO2 environmental chamber.
TIRF (Single Molecule Super-resolution)
Olympus IX83 inverted stand with custom focus-lock. 3 excitation lasers, XYZ piezo stage (ASI), and microfluidic Aria for multiplexed imaging (DNA-PAINT).
Nikon Spinning Disk (All-purpose workhorse)
Nikon Ti-Eclipse with Yokogawa CSU-X spinning disk unit and perfect focus. Features 5 excitation lasers, fast 3D imaging, TIRF, FRAP, and an environmental chamber.
Deltavision Wide-Field Microscope (2D Tissue Dynamics)
Equipped with autofocus, temperature and CO2 box, and an automated plate reader for screening applications.
In addition, we have access to the CMCB light microscopy facility.
Cell Culture & Genetic Engineering
We use immortalized and stem cell lines for our research. We use CRISPR to produce KO and fluorescent KI cell lines. We use inducible protein degradation systems (Degron, PROTAC) to study dynamics of KO phenotypes. Please contact us if you want to use any published cell line.
Protein Expression & Purification
We use bacterial and insect cell expression systems in combination with chromatography to produce high quality proteins for our molecular reconstitution projects.We collaborate with the Alberti Lab for protein characterization and quality control.
Wetlab
We have a well-equipped wetlab space for our molecular biology and biochemistry work.